Scientific documentation since 1981
Xinix has built up extensive scientific documentation in collaboration with universities, independent laboratories, and specialist organizations worldwide.
Over the years, the documentation has included, among others, Johns Hopkins University, London Hospital Medical College, King’s College School of Medicine & Dentistry, Singapore University, Michigan State University and Clancy Environmental Consultants.
Newer tests and analyses have been carried out by Chelab S.r.l./Mérieux NutriSciences in Italy, MICROMUN/Hygiene Nord in Germany, Melbec Microbiology in the UK, and Eurofins in Sweden. Field trials have also been conducted in collaboration with WIST Water Solutions/DrinkWell in India.
A well-documented active substance
Chlorine dioxide (ClO2) is a well-established active chemical substance that has been used for water treatment and disinfection since the 1930s, primarily in large industrial applications. The substance is recognized as one of the most effective methods for disinfecting water and surfaces. Chlorine dioxide is included in the Swedish Food Agency's regulations for drinking water preparation and is an established active substance regulated by the EU Biocidal Products Regulation.
Article 95 of the EU Biocidal Products Regulation sets specific documentation requirements for suppliers of active substances for biocidal products. Xinix's regulatory basis includes documentation of the supply chain for chlorine dioxide raw materials. Product approvals and permitted uses are evaluated separately in each market.
Documentation in brief
- Tests according to 8 EN - European Standards, which specify test methods and requirements for products, processes and services.
- Documented efficacy against, among others, viruses, bacteria, yeast, mold, mycobacteria, and spores.
- Independent water analyses using ISO-standardized methods.
- Laboratory tests, operational controls, and field trials from 1981 to today.
How to read the documentation
- Laboratory test: The product is tested under controlled conditions according to a specified method or standard.
- Operational and field control: Samples are taken in a real facility or environment. The result applies to the specific sample and time of sampling.
- Approval: A regulatory decision showing which uses and product claims have been approved in a specific market.
Testing and documentation per product area
FreeBact®Water - Disinfection of drinking water and stored water
FreeBact® Water comprises solutions for drinking water disinfection, from small quantities for personal use to stored water and larger tank volumes. The documentation includes laboratory tests, independent water analyses, and field trials in demanding water environments, including contaminated natural water, floodwater, and E. coli.
Problematic and flooded water in India
FreeBact® Water was used in field trials with sewage-affected, turbid, and flooded water. The results document practical use in demanding water environments. [Source: WIST Water Solutions/DrinkWell, India, 2024]
E. coli in contaminated natural water
Xinix was tested in discolored natural water with visible particles and a likely high organic load. The results show efficacy against E. coli under the tested conditions. [Source: Swedish Institute for Infectious Disease Control, Sweden, 2007]
Pool and SPA
Water samples from pool and spa environments were analyzed for microbiological water quality. [Source: Eurofins Water Testing Sweden, 2025]
Historical water documentation
Older studies include E. coli, cholera, Salmonella, Shigella, Giardia,
Cryptosporidium, and poliovirus. [Sources: Johns Hopkins University, Swedish Institute for Infectious Disease Control, Clancy Environmental Consultants, Wisconsin State Laboratory of Hygiene, Singapore University, KEBS, and CSIR.]
FreeBact® Dosage - Continuous disinfection of pools and water systems
FreeBact® Dosage includes systems for continuous dosing in pools, spas, and other water systems. The results below are based on municipal inspections and accredited analyses from real pool and spa facilities, focusing on microbiological water quality.
Björnrike Ski Club & Björnbadet
A municipal inspection confirms that the facility uses FreeBact® Dosage. An accredited pool sample showed less than 1 cfu/ml cultivable bacteria and less than 1 cfu/100 ml Pseudomonas aeruginosa. The levels were below the applicable guideline values. [Sources: Berg and Härjedalen Environmental and Building Committee and Hjortens Laboratorium, 2026]
Birka Gotland
FreeBact® Dosage installed January, 2025. Three pools were analyzed: indoor pool, cold bath, and outdoor pool. All samples were below the guideline values for cultivable bacteria, E. coli, intestinal enterococci, and Pseudomonas aeruginosa. [Source: Eurofins Pegasuslab, Sweden, 2025]
FreeBact® Surface -Surface Disinfection
FreeBact® Surface is used for disinfection of surfaces in many different environments. The documentation in this section includes standardized laboratory tests against bacteria, viruses, yeast and mould, as well as older tests against mycobacteria and spores, among others. The results apply to the organisms, contact times and test conditions specified in each report.
Bacteria on surfaces
FreeBact® Surface achieved at least a 4 log reduction against Staphylococcus aureus, Pseudomonas aeruginosa, E. coli and Enterococcus hirae within five minutes under clean test conditions. [Source: UNI EN 13697:2023, Chelab S.r.l./Mérieux NutriSciences, Italy, 2025]
Yeast and mould
FreeBact® Surface achieved at least a 3 log reduction against Candida albicans and Aspergillus brasiliensis within 15 minutes. [Source: UNI EN 13697:2023, Chelab S.r.l./Mérieux NutriSciences, Italy, 2025]
Poliovirus, adenovirus and murine norovirus
SanDes was tested against three specific test viruses. The report shows more than a 5 log reduction
for murine norovirus within one minute, even under high protein load. [Source:
EN 14476, MICROMUN/Hygiene Nord GmbH, Germany, 2018]
Other surface documentation
Older tests include mycobacteria, Bacillus subtilis spores,
Clostridium difficile, Candida and Aspergillus. [Sources: Hygiene Nord GmbH, MICROMUN and Örebro University Hospital]
FreeBact® Surface On-The-Go and FreeBact® Surface Industry have product approvals in Switzerland for the uses and conditions specified in the respective decisions. [Source: Federal Office of Public Health, Switzerland, decisions CHZN7674 and CHZN7676, 2025.]
FreeBact® Fogging - Disinfection of enclosed spaces
FreeBact® Fogging is a portable solution for fast and comprehensive disinfection of enclosed spaces with dry fog. The system, including Hydra LiTE and Hydra AiO, has been tested by the independent laboratory Melbec Microbiology according to BS EN 17272:2020, the standard for automated airborne room disinfection processes.
Room disinfection tested according to EN 17272
FreeBact® Fogging was tested together with Hydra LiTE and Hydra AiO in an enclosed space of 36 m³. The test was conducted by the independent laboratory Melbec Microbiology according to BS EN 17272:2020. Under clean test conditions, the system achieved a reduction of at least 5 log for bacteria and at least 4 log for yeast. The system thus met the requirements applied in the test. The test cycle included 22 minutes of treatment and 15 minutes of ventilation. [Source: BS EN 17272:2020, Melbec Microbiology, UK, 2025]
Read the FreeBact® Fogging test report according to EN 17272
Approvals and certificates
Current regulatory decisions and product approvals are presented separately from efficacy, field, and operational tests. Sources: Swiss Federal Office of Public Health,
the Swedish Food Agency and relevant documents within the EU Biocidal Products Regulation.
Documented history 1981-2026
2026
- FreeBact® Water Dosage – municipal supervision and accredited analysis of pool water, Björnrike – Berg and Härjedalen environmental and building committee / Hjorten's Laboratorium, Sweden
2025
- FreeBact® Fogging – room disinfection according to BS EN 17272:2020 – Melbec Microbiology, United Kingdom
- FreeBact® Surface – evaluation of bactericidal activity for general disinfection according to UNI EN 13697:2023 – Chelab S.r.l./Mérieux NutriSciences, Italy
- FreeBact® Water – water analysis for pool and spa – Eurofins, Sweden
- FreeBact® Water Dosage – microbiological analyses of indoor pool, cold bath, and outdoor pool, Birka Gotland – Eurofins Pegasuslab, Sweden
- FreeBact® Surface – fungicidal and yeasticidal activity according to UNI EN 13697:2023 – Chelab S.r.l./Mérieux NutriSciences, Italy
- FreeBact® Surface – laboratory test against Legionella pneumophila in aqueous environment according to UNI EN 13623:2020 – Chelab S.r.l./Mérieux NutriSciences, Italy
- Product approvals for FreeBact® Surface On-The-Go and FreeBact® Surface Industry – Bundesamt für Gesundheit, Switzerland
2024
- DrinkWell – several field trials with flooded and problematic water in rural areas of India
2018
- Tested against poliovirus type 1, adenovirus 5/75 and murine norovirus according to EN 14476 – MICROMUN/Hygiene Nord GmbH, Germany
- Bactericidal activity against Enterococcus hirae according to EN 13727 – Hygiene Nord GmbH, Germany
- Bactericidal activity against Staphylococcus aureus according to EN 13727 – Hygiene Nord GmbH, Germany
- Bactericidal activity against Pseudomonas aeruginosa according to EN 13727 – Hygiene Nord GmbH, Germany
- Fungicidal activity against Aspergillus brasiliensis according to EN 13624 – Hygiene Nord GmbH, Germany
- Yeasticidal activity against Candida albicans according to EN 13624 – Hygiene Nord GmbH, Germany
- Sporicidal activity against Bacillus subtilis according to EN 13704 – Hygiene Nord GmbH, Germany
- Tuberculocidal and mycobactericidal activity according to EN 14348 – Hygiene Nord GmbH, Germany
2016
- Water Disinfection – Kenya Bureau of Standards (KEBS), Kenya
- Water Disinfection – ONEP, Abidjan, Côte d'Ivoire
2010
- Historical test against Clostridium difficile – Örebro University Hospital, Sweden
- Historical test of hand disinfectant product – MICAN Lab, Mölnlycke, Sweden
2009
- Spore-forming bacteria on surfaces – Örebro University Hospital, Sweden
2008
- Spore-forming bacteria, including Clostridium difficile – Örebro University Hospital, Sweden
- E. coli in contaminated water – Swedish Institute for Infectious Disease Control, Sweden
- Analysis of trihalomethanes (THM) after treatment with XiniX and hypochlorite, respectively – ALS Scandinavia
2007
- Spore-forming bacteria – Battelle Memorial Institute, USA
2006
- Study in dentistry – Dental Surgery, Pittsburgh, USA
- Water Disinfection – USACHPPM, USA
2005
- Surface tension in surface disinfection – electron microscope study, Sweden
2002
- Giardia and Cryptosporidium cysts – Swedish Institute for Infectious Disease Control, Sweden
2001
- Bacillus subtilis (spores) – SIK, Institute for Food and Biotechnology, Sweden
- Poliovirus, Rotavirus W.a and Klebsiella terrigena – Clancy Environmental Consultants
- Poliovirus – Wisconsin State Laboratory of Hygiene, USA
2000
- E. coli and other bacteria –
Johns Hopkins University, USA - Vibrio cholerae, Salmonella typhi, Shigella sonnei, Mycobacterium tuberculosis, Giardia lamblia and Escherichia coli – Johns Hopkins University / Universidad Peruana Cayetano Heredia, Peru
1999
- Halitosis (bad breath) and caries inhibition – Odontology, University of Gothenburg, Sweden
1998
- Water Disinfection – The Water Programme, Environmentek/CSIR, Kenya
1996
- Analysis of residues – KM Lab AB, Sweden
- Historical product approval – National Institute of Hygiene, Poland
- Stability test – KM Lab AB, Halmstad, Sweden
1994
- E. coli and other bacteria – Singapore University
- Oral hygiene studies – London Hospital Medical College Dental School and King’s College School of Medicine & Dentistry, London
1993
- Corrosion test – Scientific Material International, Inc., USA
1992
- Escherichia coli – University of Gothenburg, Sweden
1989
- Escherichia coli – National Bacteriological Laboratory (SBL), Sweden
1981
- Stability test – Water Laboratory, Sweden
