Updated Oct 2026 (v 4.2)

Scientific documentation since 1981

Xinix has built up extensive scientific documentation together with universities, independent laboratories, and specialist organizations worldwide. 

Over the years, the documentation has included, among others, Johns Hopkins University, London Hospital Medical College, King’s College School of Medicine & Dentistry, Singapore University, Michigan State University and Clancy Environmental Consultants.

More recent tests and analyses have been carried out by Chelab S.r.l./Mérieux NutriSciences in Italy, MICROMUN/Hygiene Nord in Germany, Melbec Microbiology in the United Kingdom, and Eurofins in Sweden. Field trials have also been carried out together with WIST Water Solutions/DrinkWell in India. 

During follow-up Legionella sampling at a property company in Norrland, analyses carried out by Eurofins showed a reduction in Legionella bacteria of between 90 and 98.8% after treatment with FreeBact® Dosage.

A well-documented active substance
Chlorine dioxide (ClO2) is a well-established active chemical substance that has been used for water treatment and disinfection since the 1930s, primarily in large-scale industrial applications. The substance is recognized as one of the most effective methods for the disinfection of water and surfaces. Chlorine dioxide is included in the Swedish Food Agency's regulations for the preparation of drinking water and is an established active substance regulated by the EU Biocidal Products Regulation. 

Article 95 of the EU Biocidal Products Regulation sets special documentation requirements for suppliers of active substances for biocidal products. Xinix's regulatory documentation includes documentation of the supply chain for chlorine dioxide raw materials. Product approvals and permitted uses are assessed separately in each respective market.

Documentation in brief

  • Tests according to 8 EN - European Standards, which specify test methods and requirements for products, processes and services.
  • Documented efficacy against, among others, viruses, bacteria, yeast, mold, mycobacteria, and spores.
  • Independent water analyses using ISO-standardized methods.
  • Laboratory tests, operational controls, and field trials from 1981 to today.

How to read the documentation

  • Laboratory test: The product is tested under controlled conditions according to a specified method or standard.
  • Operational and field control: Samples are taken in a real facility or environment. The result applies to the specific sample and time of sampling.
  • Approval: A regulatory decision showing which uses and product claims have been approved in a specific market.

Testing and documentation per product area

FreeBact®Water - Disinfection of drinking water and stored water

FreeBact® Water covers solutions for drinking water disinfection, from small quantities for personal use to stored water and larger tank volumes. The documentation includes laboratory tests, independent
water analyses and field trials in demanding water environments, including contaminated natural water, floodwater and E. coli.


Problematic and floodwater in India
FreeBact® Water was used in field trials with sewage-contaminated, turbid and floodwater. The results document practical use in demanding water environments. [Source: WIST Water Solutions/DrinkWell, India, 2024]

E. coli in contaminated natural water
Xinix was tested in discolored natural water with visible particles and likely high organic load. The results show effectiveness against E. coli under the tested conditions [Source: Swedish Public Health Agency, Sweden, 2007]

Pool and SPA
Water samples from pool and spa environments were analyzed for microbiological water quality. [Source: Eurofins Water Testing Sweden, 2025]

Historical water documentation
Older studies include, among others, E. coli, cholera, Salmonella, Shigella, Giardia,
Cryptosporidium and poliovirus. [Sources: Johns Hopkins University, Swedish Public Health Agency, Clancy Environmental Consultants, Wisconsin State Laboratory of Hygiene, Singapore University, KEBS and CSIR.]

FreeBact® Dosage - Continuous disinfection of pools and water systems

FreeBact® Dosage includes systems for continuous dosing in buildings, pools, spas, and other water systems. The results below are based on sampling and analyses from real-world installations, with a focus on microbiological water quality.

Property Management Company, Norrland
Follow-up sampling in the building's water system showed a clear reduction in bacterial levels. Hot and cold water samples measured 10 cfu/1000 ml, while a sample taken after stagnation showed 150 cfu/1000 ml. Compared to previous levels, the results corresponded to a reduction of approximately 90–98.8%. [Source: test results, 2026 sv | eng]

Björnrike Ski Club & Björnbadet
A municipal inspection confirms that the facility uses FreeBact® Dosage. An accredited pool sample showed fewer than 1 cfu/ml culturable bacteria and fewer than 1 cfu/100 ml Pseudomonas aeruginosa. The levels were below the applicable guidelines. [Sources: Berg och Härjedalen Environmental and Building Committee and Hjortens Laboratorium, 2026]

Birka Gotland
FreeBact® Dosage installed January 2025. Three pools were analysed: indoor pool, cold plunge pool, and outdoor pool. All samples were below the guidelines for culturable bacteria, E. coli, intestinal enterococci, and Pseudomonas aeruginosa. [Source: Eurofins Pegasuslab, Sweden, 2025]



 

FreeBact® Surface -Surface Disinfection

FreeBact® Surface is used for surface disinfection in many different environments. The documentation in this section includes standardized laboratory tests against bacteria, viruses, yeast, and mold, as well as older tests against, among others, mycobacteria and spores. The results apply to the organisms, contact times, and test conditions specified in the respective report. 

Bacteria on surfaces
FreeBact® Surface achieved at least a 4 log reduction against Staphylococcus aureus, Pseudomonas aeruginosa, E. coli, and Enterococcus hirae within five minutes under clean test conditions. [Source: UNI EN 13697:2023, Chelab S.r.l./Mérieux NutriSciences, Italy, 2025]

Yeast and mold
FreeBact® Surface achieved at least a 3 log reduction against Candida albicans and Aspergillus brasiliensis within 15 minutes. [Source: UNI EN 13697:2023, Chelab S.r.l./Mérieux NutriSciences, Italy, 2025]

Poliovirus, adenovirus, and murine norovirus
SanDes was tested against three specific test viruses. The report shows more than a 5 log reduction
for murine norovirus within one minute, even under high protein load. [Source:
EN 14476, MICROMUN/Hygiene Nord GmbH, Germany, 2018]

Other surface documentation
Older tests include, among others, mycobacteria, Bacillus subtilis spores,
Clostridium difficile, Candida, and Aspergillus. [Sources: Hygiene Nord GmbH, MICROMUN, and Örebro University Hospital]

FreeBact® Surface On-The-Go and FreeBact® Surface Industry have product approvals in Switzerland for the areas of use and conditions specified in the respective decision. [Source: Federal Office of Public Health, Switzerland, decisions CHZN7674 and CHZN7676, 2025.]

FreeBact® Fogging - Disinfection of enclosed spaces

FreeBact® Fogging is a portable solution for rapid and comprehensive disinfection of enclosed spaces using dry fog. The system with Hydra LiTE and Hydra AiO has been tested by the independent laboratory Melbec Microbiology in accordance with BS EN 17272:2020, the standard for automated processes for airborne room disinfection.

Room disinfection tested according to EN 17272
FreeBact® Fogging was tested together with Hydra LiTE and Hydra AiO in an enclosed space of 36 m³. The test was carried out by the independent laboratory Melbec Microbiology in accordance with BS EN 17272:2020. Under controlled test conditions, the system achieved a reduction of at least 5 log for bacteria and at least 4 log for yeast. The system thus met the requirements applied in the test. The test cycle included 22 minutes of treatment and 15 minutes of ventilation. [Source: BS EN 17272:2020, Melbec Microbiology, United Kingdom, 2025]
Read the test report for FreeBact® Fogging according to EN 17272

Approvals and certificates
Current regulatory decisions and product approvals are presented separately from efficacy, field, and operational tests. Sources: Swiss Federal Office of Public Health,
Swedish Food Agency and relevant documents within the EU biocidal regulations.



Documented history 1981-2026

2026

  • FreeBact® Water Dosage – municipal inspection and accredited analysis of swimming pool water, Björnrike – Berg and Härjedalen Environmental and Building Committee / Hjortens Laboratorium, Sweden

2025

  • FreeBact® Fogging – room disinfection according to BS EN 17272:2020 – Melbec Microbiology, United Kingdom
  • FreeBact® Surface – evaluation of bactericidal activity for general disinfection according to UNI EN 13697:2023 – Chelab S.r.l./Mérieux NutriSciences, Italy
  • FreeBact® Water – water analyses for pool and spa – Eurofins, Sweden
  • FreeBact® Water Dosage – microbiological analyses of indoor pool, cold plunge pool and outdoor pool, Birka Gotland – Eurofins Pegasuslab, Sweden
  • FreeBact® Surface – antifungal and anti-yeast activity according to UNI EN 13697:2023 – Chelab S.r.l./Mérieux NutriSciences, Italy
  • FreeBact® Surface – laboratory test against Legionella pneumophila in water according to UNI EN 13623:2020 – Chelab S.r.l./Mérieux NutriSciences, Italy
  • Product approvals for FreeBact® Surface On-The-Go and FreeBact® Surface Industry – Federal Office of Public Health, Switzerland

2024

  • DrinkWell – several field trials with flooded and problematic water in rural areas in India

2018

  • Tested against poliovirus type 1, adenovirus 5/75 and murine norovirus according to EN 14476 – MICROMUN/Hygiene Nord GmbH, Germany
  • Bactericidal activity against Enterococcus hirae according to EN 13727 – Hygiene Nord GmbH, Germany
  • Bactericidal activity against Staphylococcus aureus according to EN 13727 – Hygiene Nord GmbH, Germany
  • Bactericidal activity against Pseudomonas aeruginosa according to EN 13727 – Hygiene Nord GmbH, Germany
  • Antifungal activity against Aspergillus  brasiliensis according to EN 13624 – Hygiene Nord GmbH, Germany
  • Anti-yeast activity against Candida albicans according to EN 13624 – Hygiene Nord GmbH, Germany
  • Sporicidal activity against Bacillus subtilis according to EN 13704 – Hygiene Nord GmbH, Germany
  • Tuberculocidal and mycobactericidal activity according to EN 14348 – Hygiene Nord GmbH, Germany

2016

  • Water disinfection – Kenya Bureau of Standards (KEBS), Kenya
  • Water disinfection – ONEP, Abidjan, Ivory Coast

2010

  • Historical test against Clostridium difficile – Örebro University Hospital, Sweden
  • Historical test of hand disinfection product – MICAN Lab, Mölnlycke, Sweden

2009

  • Spore-forming bacteria on surfaces – Örebro University Hospital, Sweden

2008

  • Spore-forming bacteria, including Clostridium difficile – Örebro University Hospital, Sweden
  • E. coli in contaminated water – Swedish National Board of Health and Welfare, Sweden
  • Analysis of trihalomethanes (THM) after treatment with XiniX and hypochlorite respectively – ALS Scandinavia

2007

  • Spore-forming bacteria – Battelle Memorial Institute, USA

2006

  • Dental study – Dental Surgery, Pittsburgh, USA
  • Water disinfection – USACHPPM, USA

2005

  • Surface tension during surface disinfection – electron microscope study, Sweden

2002

  • Giardia and Cryptosporidium cysts – Swedish National Board of Health and Welfare, Sweden

2001

  • Bacillus subtilis (spores) – SIK, Swedish Institute for Food and Biotechnology, Sweden
  • Poliovirus, Rotavirus W.a and Klebsiella terrigena – Clancy Environmental Consultants
  • Poliovirus – Wisconsin State Laboratory of Hygiene, USA

2000

  • E. coli and other bacteria –
    Johns Hopkins University, USA
  • Vibrio cholerae, Salmonella typhi, Shigella sonnei, Mycobacterium tuberculosis, Giardia lamblia and Escherichia coli – Johns Hopkins University / Universidad Peruana Cayetano Heredia, Peru

1999

  • Halitosis (bad breath) and caries prevention – Odontologen, University of Gothenburg, Sweden

1998

  • Water disinfection – The Water Programme, Environmentek/CSIR, Kenya

1996

  • Analysis of residual chlorine – KM Lab AB, Sweden
  • Historical product approval – National Institute of Hygiene, Poland
  • Stability test – KM Lab AB, Halmstad, Sweden

1994

  • E. coli and other bacteria – Singapore University 
  • Studies in oral hygiene – London Hospital Medical College Dental School and King’s College School of Medicine & Dentistry, London

1993

  • Corrosion test – Scientific Material International, Inc., USA

1992

  • Escherichia coli – University of Gothenburg, Sweden

1989

  • Escherichia coli – State Bacteriological Laboratory (SBL), Sweden

1981

  • Stability test – Water Laboratory, Sweden 
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